Quantitative analysis of the human semen phosphorometabolome by 31P-NMR

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dc.contributor.authorSerrano Pérez, Rebeca
dc.contributor.authorMartín Hidalgo, Martín
dc.contributor.authorBilbao García, Jon
dc.contributor.authorBernardo Seisdedos, Ganeko
dc.contributor.authorMillet, Óscar
dc.contributor.authorGarcía Marín, Luis Jesús
dc.contributor.authorBragado González, María Julia
dc.date.accessioned2025-05-20T14:03:09Z
dc.date.available2025-05-20T14:03:09Z
dc.date.issued2024-01-30
dc.date.updated2025-05-20T14:03:09Z
dc.description.abstractPhosphorus-containing metabolites occupy a prominent position in cell pathways. The phosphorometabolomic approach in human sperm samples will deliver valuable information as new male fertility biomarkers could emerge. This study analyzed, by 31P-NMR, seminal plasma and whole semen from asthenozoospermic and normozoospermic samples (71% vs. 27% and 45% vs. 17%, total and progressive sperm motility, respectively), and also ejaculates from healthy donors. At least 16 phosphorus-containing metabolites involved in central energy metabolism and phospholipid, nucleotide, and nicotinamide metabolic pathways were assigned and different abundances between the samples with distinct sperm quality was detected. Specifically, higher levels of phosphocholine, glucose-1-phosphate, and to a lesser degree, acetyl phosphate were found in the asthenozoospermic seminal plasma. Notably, the phosphorometabolites implicated in lipid metabolism were highlighted in the seminal plasma, while those associated with carbohydrate metabolism were more abundant in the spermatozoa. Higher levels of phosphocholine, glucose-1-phosphate, and acetyl phosphate in the seminal plasma with poor quality suggest their crucial role in supporting sperm motility through energy metabolic pathways. In the seminal plasma, phosphorometabolites related to lipid metabolism were prominent; however, spermatozoa metabolism is more dependent on carbohydrate-related energy pathways. Understanding the presence and function of sperm phosphorylated metabolites will enhance our knowledge of the metabolic profile of healthy human sperm, improving assessment and differential diagnosis.en
dc.description.sponsorshipThis research was funded by the regional Grant from Junta de Extremadura (Spain) IB20154 and GR18094en
dc.identifier.citationSerrano, R., Martin-Hidalgo, D., Bilbao, J., Bernardo-Seisdedos, G., Millet, O., Garcia-Marin, L. J., & Bragado, M. J. (2024). Quantitative analysis of the human semen phosphorometabolome by 31P-NMR. International Journal of Molecular Sciences, 25(3). https://doi.org/10.3390/IJMS25031682
dc.identifier.doi10.3390/IJMS25031682
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://hdl.handle.net/20.500.14454/2785
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rights© 2024 by the authors
dc.subject.otherAsthenozoospermia
dc.subject.otherHuman sperm
dc.subject.otherMetabolomics
dc.subject.otherNMR-based phosphoromics
dc.subject.otherSeminal plasma
dc.titleQuantitative analysis of the human semen phosphorometabolome by 31P-NMRen
dc.typejournal article
dcterms.accessRightsopen access
oaire.citation.issue3
oaire.citation.titleInternational Journal of Molecular Sciences
oaire.citation.volume25
oaire.licenseConditionhttps://creativecommons.org/licenses/by/4.0/
oaire.versionVoR
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